Plasmid: DNA separate from the chromosomal DNA and capable of independent replication. Found naturally in many bacteria, it is typically circular and double-stranded.

 

Related Words

Plasmid: DNA separate from the chromosomal DNA and capable of independent replication. Found naturally in many bacteria, it is typically circular and double-stranded.

Plasmids: Self-replicating, circular DNA molecules found in bacterial cells; often used as vectors in recombinant DNA technology. Small circles of double-stranded DNA found in some bacteria. Plasmids . . . View Full Definition

R-Plasmid: A plasmid carrying genes controlling resistance to various drugs.

Gene Mapping: Determination of the relative positions of genes on a dna molecule (chromosome or plasmid) and of the distance, in linkage units or physical units, between them.

Pku: Phenylketonuria, an enzyme deficiency condition characterized by the inability to convert one amino acid, phenylalanine, to another, tyrosine, resulting in mental deficiency. Plasmid double- . . . View Full Definition

Cosmid: Artificially constructed cloning vector containing the cos gene of phage lambda. Cosmids can be packaged in lambda phage particles for infection into e. Coli: this permits cloning of larger . . . View Full Definition

Bacterial Artificial Chromosome (BAC): A vector used to clone dna fragments (100- to 300-kb insert size: average, 150 kb) in escherichia coli cells. Based on naturally occurring f-factor plasmid found in the bacterium e. Coli.

Recombinant Dna Molecules: New combinations of DNA fragments formed by cutting DNA segments from two sources with restriction enzyme and then joining the fragments together with DNA ligase. Interspecies transfer of ge . . . View Full Definition

Restriction Enzymes: A series of enzymes that attach to DNA molecules at specific nucleotide sequences and cut both strands of DNA at those sites. A bacterial enzyme that cuts DNA at a specific recognition seque . . . View Full Definition

Cloning Vector: Dna molecule originating from a virus, a plasmid, or the cell of a higher organism into which another dna fragment of appropriate size can be integrated without loss of the vectors capacity . . . View Full Definition